iLas Ring-TIRF Microscopes
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We have two imaging systems featuring听iLasV2 FRAP/Ring TIRF modulation. The iLas system is a unique optical system for real鈥恡ime control of laser illumination. It can perform a variety of targeted laser experiments and each microscope has specific customizations outlined below.
Common Features:
- Zeiss AxioObserver Z1 stand
- Zeiss Power Supply 232
- Zeiss Definite Focus Module
- iLasV2 FRAP/TIRF system for 360 degree TIRF, FRAP, and Photoactivation
- MetaMorph and MicroManager V2 software
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Excitation Objectives:
- 40x oil Plan-Apochromat DIC M27, NA=1.4
- 63x oil Plan-Apochromat Corr M27, NA=1.46
- 100x oil Plan-Apochromat DIC M27, NA=1.46 (x2)
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Imaging System Specifics:
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SYSTEM听I
Features:
- Stage:听Ludl MAC 6000 System from Quorum
- Lamp: X-Cite 120Q LED from Excelitas听
- Two cameras from Andor; iXon听EMCCD Ultra 897 and Neo 5.5 sCMOS
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Available Excitation Lasers:
- 488 nm, 80 mW
- 561 nm, 100 mW
- 635 nm, 140 mW
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Fluorescence Components:
- Filter #8 - DAPI
- Filter #2 - GFP, AlexaFluor488
- Filter #7 - TRITC, AlexaFluor 546
- Filter #4 - AlexaFluor635
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Optional Incubation Systems:
- Objective heater: TempController 2000-2 by Pecon
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SYSTEM听II
Features:
- Stage: ASI听MS-2000-500 XYZ Automated Stage
- Lamp: PhotoFluor II from听89 North
- Dual cameras from Photometrics; two Prime 95B sCMOS and TwinCam LS Image Splitter 1.0x from Cairn
- Single camera from Andor; iXon EMCCD Ultra 897
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Available Excitation Lasers:
- 405 nm, 100 mW
- 445 nm, 75 mW
- 491 nm, 100 mW
- 514 nm, 40 mW
- 561 nm, 100 mW
- 635 nm, 140 mW
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Fluorescence Components:
- Filter #10 - DAPI
- Filter #1, #3 - GFP, AlexaFluor488
- Filter #5, #6 - AlexaFluor546
- Filter #9 - AlexaFluor635
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Optional Incubation Systems:
- Objective heater: CU-501 temperature controller by Live Cell Instrument听
- CO2 Support: FC-5N Air Mixer by Live Cell Instrument
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