平特五不中

Anyone who has ever tried to put on a Band-Aid^庐 when their skin is damp knows that it can be frustrating. Wet skin isn鈥檛 the only challenge for medical adhesives - the human body is full of blood, serum, and other fluids that complicate the repair of numerous internal injuries. Many of the adhesive products used today are toxic to cells, inflexible when they dry, and do not bind strongly to biological tissue. But researchers from Harvard and 平特五不中 universities have created a super-strong 鈥渢ough adhesive鈥� that is biocompatible and binds to tissues with a strength comparable to the body鈥檚 own resilient cartilage, even when they鈥檙e wet. The research is reported in this week鈥檚 issue of Science.

When first author Jianyu Li, (then a postdoctoral fellow at Harvard鈥檚 Wyss Institute for Biologically Inspired Engineering and now an Assistant Professor at 平特五不中鈥檚 Department of Mechanical Engineering) started thinking about how to improve medical adhesives, he found a solution in an unlikely place: a slug. The Dusky Arion (Arion subfuscus), common in Europe and parts of the United States, secretes a special kind of mucus when threatened that glues it in place, making it difficult for a predator to pry it off its surface. This glue was previously determined to be composed of a tough matrix peppered with positively charged proteins, which inspired Li and his colleagues at the Wyss Institute and Harvard鈥檚 John A. Paulson School of Engineering and Applied Sciences (SEAS) to create a double-layered hydrogel consisting of an alginate-polyacrylamide matrix supporting an adhesive layer that has positively-charged polymers protruding from its surface.

Key feature

The polymers bond to biological tissues via three mechanisms - electrostatic attraction to negatively charged cell surfaces, covalent bonds between neighboring atoms, and physical interpenetration - making the adhesive extremely strong. But the matrix layer is equally important, says Li: 鈥淢ost prior material designs have focused only on the interface between the tissue and the adhesive. Our adhesive is able to dissipate energy through its matrix layer, which enables it to deform much more before it breaks.鈥� The team鈥檚 design for the matrix layer includes calcium ions that are bound to the alginate hydrogel via ionic bonds. When stress is applied to the adhesive, those 鈥渟acrificial鈥� ionic bonds break first, allowing the matrix to absorb a large amount of energy before its structure becomes compromised.

鈥淭he key feature of our material is the combination of a very strong adhesive force and the ability to transfer and dissipate stress, which have historically not been integrated into a single adhesive,鈥� says corresponding author Dave Mooney, Ph.D., who is a founding Core Faculty member at the Wyss Institute and the Robert P. Pinkas Family Professor of Bioengineering at SEAS.

The researchers tested their adhesive on a variety of both dry and wet pig tissues including skin, cartilage, heart, artery, and liver, and found that it bound to all of them with significantly greater strength than other medical adhesives. The tough adhesive also maintained its stability and bonding when implanted into rats for two weeks, or when used to seal a hole in a pig heart that was mechanically inflated and deflated and then subjected to tens of thousands of cycles of stretching. Additionally, it caused no tissue damage or adhesions to surrounding tissues when applied to a liver hemorrhage in mice - side effects that were observed with both super glue and a commercial thrombin-based adhesive.

Potential applications

Such a high-performance material has numerous potential applications in the medical field, either as a patch that can be cut to desired sizes and applied to tissue surfaces or as an injectable solution for deeper injuries. It can also be used to attach medical devices to their target structures, such as an actuator to support heart function. 鈥淭his family of tough adhesives has wide-ranging applications,鈥� says co-author Adam Celiz, Ph.D., who is now a Lecturer at the Department of Bioengineering, Imperial College London. 鈥淲e can make these adhesives out of biodegradable materials, so they decompose once they鈥檝e served their purpose. We could even combine this technology with soft robotics to make sticky robots, or with pharmaceuticals to make a new vehicle for drug delivery.鈥�

鈥淣ature has frequently already found elegant solutions to common problems; it鈥檚 a matter of knowing where to look and recognizing a good idea when you see one,鈥� says Wyss Founding Director Donald Ingber, who is also the Judah Folkman Professor of Vascular Biology听at Harvard Medical School and the Vascular Biology Program at Boston Children鈥檚 Hospital, as well as a Professor of Bioengineering at Harvard鈥檚 School of Engineering and Applied Sciences. 鈥淲e are excited to see how this technology, inspired by a humble slug, might develop into a new technology for surgical repair and wound healing.鈥�

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Tough adhesives for diverse wet, by J. Li et al.,

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This research was funded by the Wyss Institute at Harvard University, NSF, MRSEC at Harvard University, NIH, Marie Curie International Outgoing Fellowship, Science Foundation Ireland, and Tsinghua University.