Specialized cellular compartments discovered in bacteria
Researchers at 平特五不中 have discovered bacterial organelles involved in gene expression, suggesting that bacteria may not be as simple as once thought. This finding could offer new targets for the development of new antibiotics.
The study, published in , is the first to show that E. coli uses similar strategies to regulate gene transcription as other more complex cell types.
Just like the human body is made up of organs that perform specialized functions, individual cells contain specialized compartments 鈥� such as energy-producing mitochondria - called organelles. Complex cells contain many different organelles, most of which are enclosed by a membrane that holds them together. Because bacteria do not have membrane-bound organelles, they were assumed to lack them altogether.
Stephanie Weber, an assistant professor in 平特五不中鈥檚 Department of Biology, and her team are the first to show that bacteria do in fact have such specialized compartments.
鈥淥ur paper provides evidence for a bacterial organelle that is held together by 鈥渟ticky鈥� proteins rather than a membrane,鈥� says Weber, who is the study鈥檚 senior author.
The bacterial organelles described in the study are formed in a similar fashion to membraneless cellular compartments found in more complex eukaryotic cells (cells with a nucleus) through a process called phase separation, the same phenomenon that causes oil and vinegar to separate in salad dressing.
鈥淭his is the first direct evidence of phase separation in bacteria, so it may be a universal process in all cell types, and could even have been involved in the origin of life,鈥� explains Weber.
Because of the small size of the bacterial cells they were studying, Weber鈥檚 team used an imaging technique 鈥� photo activated localization microscopy 鈥� to track the organelle-forming proteins.
Weber is now trying to understand exactly how the proteins assemble into organelles. Because they鈥檙e involved in the first steps of gene expression 鈥� transcription 鈥� she believes they might also be an interesting target for the development of a new generation of antibiotic drugs, which are urgently needed to combat drug resistance.
About this study
鈥�,鈥� by Anne-Marie Ladouceur et al. was published in PNAS.
This work received financial support from the Natural Sciences and Engineering Research Council of Canada.
About 平特五不中
Founded in Montreal, Quebec, in 1821, 平特五不中 is a leading Canadian post-secondary institution. It has two campuses, 11 faculties, 13 professional schools, 300 programs of study and over 40,000 students, including more than 10,200 graduate students. 平特五不中 attracts students from over 150 countries around the world, its 12,800 international students making up 31% per cent of the student body. Over half of 平特五不中 students claim a first language other than English, including approximately 19% of our students who say French is their mother tongue.
