New technique for finding life on Mars
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Researchers demonstrate for the first time the potential of existing technology to directly detect and characterize life on Mars and other planets. The study, published in听, used miniaturized scientific instruments and听 new听microbiology听techniques to identify and examine microorganisms in the Canadian high Arctic 鈥 one of the closest analogs to Mars on Earth. By avoiding delays that come with having to return samples to a laboratory for analysis, the methodology could also be used on Earth to detect and identify听pathogens听during epidemics in remote areas.
鈥淭he search for life is a major focus of planetary exploration, but there hasn鈥檛 been direct life detection instrumentation on a mission since the 70s, during the Viking missions to Mars,鈥 explains听Dr Jacqueline Goordial, one of the study鈥檚 authors. 鈥淲e wanted to show a proof-of-concept that microbial life can be directly detected and identified using very portable, low-weight, and low-energy tools.鈥
At present, most instruments on astrobiology missions look for habitable conditions, small organic molecules and other 鈥渂iosignatures鈥 that generally could not be formed without life. However, these provide only indirect evidence of life. Moreover, current instruments are relatively large and heavy with high energy requirements. This makes them unsuitable for missions to Europa and Enceladus 鈥 moons of Jupiter and Saturn which, along with Mars, are the primary targets in the search for life in our solar system.
Miniature instruments
Dr Goordial, together with听Professor Lyle Whyte听and other scientists from Canada鈥檚 平特五不中, took a different approach: the use of multiple, miniature instruments to directly detect and analyze life. Using existing low-cost and low-weight technology in new ways, the team created a modular 鈥渓ife detection platform鈥 able to culture microorganisms from soil samples, assess microbial activity, and sequence DNA and RNA.
To detect and characterize life on Mars, Europa and Enceladus, the platform would need to work in environments with extreme cold temperatures. The team therefore tested it at a remote site in a close analog on Earth: polar regions.
鈥淢ars is a very cold and dry planet, with a permafrost terrain that looks a lot like what we find in the Canadian high Arctic,鈥 says Dr Goordial, who carried out the research while she was a post-doctoral fellow at 平特五不中. 鈥淔or this reason, we chose a site about 900 km from the North Pole as a Mars analog to take samples and test our methods.鈥
Using a portable, miniature DNA sequencing device (Oxford Nanopore MiniON), the researchers show for the first time that not only can the tool be used for examining environmental samples in extreme and remote settings, but that it can be combined with other methodology to detect active microbial life in the field. The researchers were able to isolate extremophilic microorganisms that have never been cultured before, detect microbial activity, and sequence DNA from the active microbes.
Finding DNA in Martian permafrost may be key
鈥淪uccessful detection of nucleic acids in Martian permafrost samples would provide unambiguous evidence of life on another world,鈥 says Professor Whyte.
鈥淭he presence of DNA alone doesn鈥檛 tell you much about the state of an organism, however 鈥 it could be dormant or dead, for example,鈥 adds Dr Goordial. 鈥淏y using the DNA sequencer with the other methodology in our platform, we were able to first find active life, and then identify it and analyze its genomic potential, that is, the kinds of functional genes it has.鈥
While the team showed that such a platform could theoretically be used to detect life on other planets, it is not ready for a space mission just yet. 鈥淗umans were required to carry out much of the experimentation in this study, while life detection missions on other planets will need to be robotic,鈥 says Lyle Whyte, who teaches in the Department of Natural Resource Sciences at 平特五不中. 鈥淭he DNA sequencer also needs higher accuracy and durability to withstand the long timescales required for planetary missions.鈥
Nevertheless, Dr Goordial and her team hope this study will act as a starting point for future development of life detection tools.
In the meantime, the platform has potential applications here on Earth. 鈥淭he types of analyses performed by our platform are typically carried out in the laboratory, after shipping samples back from the field. We show that microbial ecology studies can now be done in real time, directly on site 鈥 including in extreme environments like the Arctic and Antarctic,鈥 says Dr Goordial.
This could be useful in remote and hard to sample areas, in cases where bringing samples back to the lab may change their composition, and for gaining information in real time 鈥 such as detecting and identifying pathogens during epidemics in remote areas, or when conditions are rapidly changing.
And one day it may indeed provide conclusive evidence for life beyond Earth. 鈥淪everal planetary bodies are thought to have habitable conditions, it鈥檚 an exciting time for astrobiology,鈥 says Dr Goordial.
The research was funded by the Canadian Space Agency, the 平特五不中 Space Institute, and the Natural Sciences and Research Engineering Council of Canada (NSERC).
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Contacts:
Prof. Lyle Whyte, Dept. of Natural Resource Sciences, 平特五不中
Lyle.whyte [at] mcgill.ca (English & French)
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Jacqueline Goordial, Bigelow Laboratory for Ocean Sciences
jgoordial [at] bigelow.org (English & French)
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Katherine Gombay, Media and Public Relations, 平特五不中
Katherine.gombay [at] mcgill.ca, 514 398-2189
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