/tropmed/aggregator J.D. MacLean Centre for Tropical & Geographic Medicine - aggregated feeds en https://pubmed.ncbi.nlm.nih.gov/39709020/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241222003411&v=2.18.0.post9+e462414 There is growing scientific and regulatory interest in transcriptomic points of departure (tPOD) values from high-throughput in vitro experiments. To further help democratize tPOD research, here we outline 'TPD-seq' which links microplate-based exposure methods involving cell lines for human (Caco-2, Hep G2) and environmental (rainbow trout RTgill-W1) health, with a commercially available RNA-seq kit, with a cloud-based bioinformatics tool (ExpressAnalyst.ca). We applied the TPD-seq workflow to... Sat, 21 Dec 2024 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/39665976/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241213002141&v=2.18.0.post9+e462414 CONCLUSIONS: Early life undernutrition may be associated with lower induction or persistence of antibody responses to certain vaccines. Addressing child undernutrition may improve vaccine efficacy and reduce the burden of vaccine-preventable diseases. Thu, 12 Dec 2024 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/39640808/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241207012056&v=2.18.0.post9+e462414 Trypanosomatids are the causative agents of deadly diseases in humans and livestock. Given the high phylogenetic distance of trypanosomatids from model organisms, these organisms have ample unannotated genes. Manual functional annotation is time-consuming, highlighting the importance of automated functional annotation tools. The development of automated functional tools is a hot research topic, and multiple tools have been developed for the task. PANNZER2 is an automated functional annotation... Fri, 06 Dec 2024 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/39631058/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241205010322&v=2.18.0.post9+e462414 CONCLUSIONS: The intervention attained targets across all studied implementation outcomes. Qualitative findings highlighted the importance of stakeholder engagement. Telehealth tools have potential for the remote follow-up of acute health conditions. Wed, 04 Dec 2024 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/39595547/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241128004242&v=2.18.0.post9+e462414 The model organism Caenorhabditis elegans and its relationship with the gut microbiome are gaining traction, especially for the study of neurodegenerative diseases such as Parkinson's Disease (PD). Gut microbes are known to be able to alter kynurenine metabolites in the host, directly influencing innate immunity in C. elegans. While the mitochondrial unfolded protein response (UPR^(mt)) was first characterized in C. elegans in 2007, its relevance in host-microbiome interactions has only become... Wed, 27 Nov 2024 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/39584985/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241126041309&v=2.18.0.post9+e462414 Over the last few decades, malaria-derived extracellular vesicles (EVs) have gained increasing interest due to their role in disease pathophysiology and parasite biology. Unlike other EV research fields, the isolation of malaria EVs is not standardized, hampering inter-study comparisons. Most malaria EV studies isolate vesicles by the "gold-standard" technique of differential (ultra)centrifugation (DC). Here, we describe in detail an optimized and reproducible protocol for the isolation of... Mon, 25 Nov 2024 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/39579617/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241125020112&v=2.18.0.post9+e462414 BACKGROUND: The most recent exacerbation of COPD (ECOPD) classification criteria relies in part on changes in respiratory rate (RR), heart rate (HR) and oxygen saturation (SpO(2)). Despite this paradigm shift, a thorough understanding of exacerbation patterns is still lacking, as is the identification of physiological exacerbation biomarkers. Sat, 23 Nov 2024 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/39556840/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241119004349&v=2.18.0.post9+e462414 No abstract Mon, 18 Nov 2024 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/39521792/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241114002954&v=2.18.0.post9+e462414 Slow progress towards implementation of conventional clinical bacteriology in low resource settings and strong interest in greater speed for antimicrobial susceptibility testing (AST) more generally has focused attention on next-generation rapid AST technologies. In this Review, we systematically synthesize publications and submissions to regulatory agencies describing technologies that provide phenotypic AST faster than conventional methods. We characterize over ninety technologies in terms of... Sat, 09 Nov 2024 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/39504434/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20241107015319&v=2.18.0.post9+e462414 CONCLUSIONS: Both LAIV3- and IIV3-elicited HA antibodies are associated with protection against influenza infection in seasons when the vaccine strains match the circulating influenza strain subtypes, supporting the use of HAI as a correlate of protection for both vaccine types in children. Wed, 06 Nov 2024 06:00:00 -0500