Ioannis Ragoussis
Professor, Department of Human Genetics
Professor Ioannis (Jiannis) Ragoussis has studied Biochemistry at the University of Tuebingen, Germany, where he obtained his PhD. He did his postdoctoral studies as EMBO fellow with J. Trowsdale at the Imperial Cancer Research Fund Laboratories in London, UK. Subsequently he became Lectures and then Senior Lecturer at the Division of Medical and Molecular Genetics at Guy鈥檚 Hospital, then Guy鈥檚 and now King鈥檚 Medical School. In 2001 he moved to the University of Oxford as Head of Genomics and in 2013 to 平特五不中 where he is Head of Genome Sciences at the 平特五不中 Genome Center and Professor in Human Genetics at the Department of Human Genetics. He is also affiliated with the Department of Bioengineering and Visiting Professor at the University of Thessaly, Greece. He has developed expertise in all fields of genomics and functional genomics. His main interest is developing state-of-the art NGS based approaches to disease gene identification,听as well as functional genomics approaches for the identification of prognostic markers and associated pathways in gynecological cancers and brain cancer.
As head of Genome Sciences, he is responsible for the Genomic Platform Development within the听平特五不中 Genome Centre. He has established the Center鈥檚 high-though put nucleic extraction facility, genotyping facility, long read sequencing technology and single cell genomics.听听He has responsibility for the scientific oversight of the MAGIC production platform, new technology evaluation and integration. As chair of the 平特五不中 Genome Center鈥檚 Technology Development and Head of the Advanced Genomic Technologies Laboratory, which includes the听Single Cell Genomics Lab of the 平特五不中 Genome Center,听he works on technical developments related to single cell genomics as well as long read sequencing technologies. In 2019 he introduced a PromethION instrument at the Center and works on applications of this technology in population studies and transcriptomics. Supported by CFI the lab includes a听Fluidigm BioMark HD听instrument, a听10X Genomics Chromium听instrument, Nanopore Sequencers (MinION听补苍诲听PromethION), a PacBio听Sequel听instrument, as well as low volume liquid handling instruments.
The Ragoussis lab is a member of 平特五不中鈥檚听Integrative and Quantitative Biology Initiative, lead by Prof. Jacalyn Vogel at the Department of Biology. The lab is responsible for single molecule sequencing using PacBio Sequel technology.
The laboratory is developing genomic tools as part of the international effort to develop Sterile Insert Techniques (SIT) in collaboration with听The International Atomic Energy Authority. As part of this work it has contributed to the groundbreaking discovery of the maleness on the Y (MoY) factor in tephretidae published in听Science:
The laboratory plays a key role in SRAS-COV-2 genome sequencing as part of the Canadian effort on Host and Virus genome sequencing, CanCOGEn, funded by Genome Canada, where Ragoussis chairs the virus genome sequencing committee.
Genomics and Functional Genomics
Single Cell Genomics: This work is in collaboration with Professors Morag Park (Tumor Heterogeneity and Microenvironment) and Richard Kremer (Circulating Tumor Cells)
Methodologies for the highly sensitive detection of both somatic as well as germline mutations: Collaboration with Will Foulkes (Inherited Breast Cancer, DICER1 Syndrome), Lucy Gilbert ( Endometrial Cancer) & Bert Vogelstein (Johns Hopkins) and Patricia Tonin (Inherited Breast and Ovarian Cancer)
The development of long read single molecule sequencing based methodologies and diagnostic tests in collaboration with Cheelong Shaw and as partner of teh igeneTRAIN Consortium (Philadeplhia Childrens Hospital)
The lab collaborates with companies such as Fluidigm, Agilent, Wafergen, with further support from Compute Canada, Department of Defence and others.
