平特五不中

As they develop, vertebrate embryos form vertebrae in a sequential, time-controlled way.聽 Scientists have determined previously that this process of body segmentation is controlled by a kind of 鈥渃lock,鈥� regulated by the oscillating activity of certain genes within embryonic cells.聽 But questions remain about how precisely this timing system works.

A new international cross-disciplinary collaboration between physicists and molecular genetics researchers advances scientists鈥� understanding of this crucial biological timing system. The study, co-authored by 平特五不中 Prof. Paul Fran莽ois and Ohio State University Prof. Sharon L. Amacher and published in聽Developmental Cell, sheds light on the clock mechanism by providing the first real-time, visual evidence of how it operates at the level of individual cells.

While previous scientific studies have examined the oscillation phenomenon in the tissue of mouse embryos, the 平特五不中 and Ohio State researchers were able to observe and analyze it in single cells. To do so, they genetically modified zebrafish 鈥� a freshwater fish whose body is nearly transparent during early development, making its anatomy easy to observe. The researchers used a fluorescent marker in the transgenic fish and developed software tools to monitor the concentration of a certain 鈥渃yclic鈥� protein, whose production rises and falls with the oscillating expression of the molecular clock genes.

It is known that cells communicate with neighboring cells through a messaging system known as the Notch signaling pathway. In their experiments with the zebrafish, the researchers cut off this inter-cellular communication network 鈥� enabling them to see how that would affect the oscillation pattern in individual cells and their neighbors.

These experiments revealed that cyclic protein concentrations in individual cells of the zebrafish continued to rise and fall, indicating that they continued to oscillate. With the inter-cellular signaling pathway blocked, however, the oscillations were no longer synchronized among neighboring cells. The cellular clocks were still ticking, in other words, but not in unison. This finding confirms that the Notch pathway serves to coordinate timing among cells 鈥� a crucial role, since the cells must act in concert in order to form vertebrae.

By observing normal zebrafish embryos, the researchers were also able to show that cells desynchronize their oscillations while performing cellular division, then later resynchronize with their neighbors as they proceed collectively to form vertebrae.

鈥淚n humans, defects in Notch signaling are associated with congenital developmental disorders called spondylocostal dysostosis, that are typified by scoliosis and trunk dwarfism caused by malformed ribs and vertebrae,鈥� Amacher notes. 鈥淪tudies such as ours may provide insight into potential therapies for human disease. It is likely that many cells in our bodies - stem cells, cancer cells - have similar molecular oscillators that regulate response to environmental signals. By unraveling such molecular clocks, we can understand how to modify them and thus change the number of oscillating cells that respond to differentiating signals, providing tremendous insight for studies in stem cell and cancer biology and tissue engineering."

鈥淭he formation of the vertebral column is very important, because everything follows from that鈥� in the development of vertebrates, Fran莽ois adds.聽 A physicist, he developed the computer tools used to analyze video footage of the zebrafish embryos. Francois鈥檚 research focuses on the modeling of physical properties of gene networks and their evolution 鈥� a field that has emerged at the nexus of biology and physics in recent years, following sequencing of the human genome and rapid growth in scientists鈥� understanding of the processes inside cells.

Fran莽ois worked with Emilie Delaune, a postdoctoral fellow who constructed the imaging tool and performed all the imaging experiments, and graduate student Nathan Shih. Amacher, Delaune and Shih conducted the research while at the University of California, Berkeley. Delaune is now with ENS Lyon in France.

Funding for the research was provided by the Association Fran莽aise contre les Myopathies, a Marie-Curie Outgoing International Fellowship, a Pew Scholar Award, the National Institutes of Health, the Natural Sciences and Engineering Research Council of Canada, and Regroupement Qu茅b茅cois pour les mat茅riaux de pointe.