Dr. Reinhardt's lab has been profiled by the 平特五不中 Reporter.
Sustainability in research: If you want to green your lab, start with the freezer
Cell biology researcher Dieter Reinhardt explains why his lab is cool with turning up the temperature of their freezers
叠测听Maya Willard-Stepan,聽Communications Intern, 平特五不中 Office of Sustainability
APRIL 6, 2023
听听听
Members of the Reinhardt lab use ultra-low temperature freezers to store reagents and other biological samples.Siddhi Aubeeluck
If you spend time in the聽, you will likely see members of the lab reach into the ultra-low freezers to access biomedical samples needed for their work. What you wouldn鈥檛 notice is that the freezer is several degrees warmer than it was in the past. In an effort to reduce the environmental impact of their research as well as the strain on their equipment, Dieter Reinhardt鈥檚 team raised the temperature of their freezers from the standard -80 degrees Celsius.
While ultra-low temperature freezers are crucial to preserving tissues, enzymes, antibodies, and other biological research samples, often the temperature is chosen聽.
Despite these machines being dubbed 鈥�-80 freezers鈥�, there is no scientific reason behind that number when it comes to maintaining the quality and safety of samples. What鈥檚 more, increasing a freezer鈥檚 temperature from -80 C to -70 C or even -65 C can聽. With hundreds of ultra-low temperature freezers at 平特五不中, this would be no small change in terms of the University鈥檚 total energy consumption and the associated financial savings.
鈥淐ompressors have to work very hard to maintain these ultra-low temperatures,鈥� said Reinhardt, PhD, and Canada Research Chair Tier 1 Laureate in Cell-Matrix Biology. 鈥淭his requires a lot of electricity, but it鈥檚 also hard on the compressors and will shorten the lifespan of your equipment, which is expensive to repair and replace. Increasing the temperature by just a few degrees can add years to this equipment and decrease how often you have to defrost it.鈥�
How cold is cold enough?
The answer isn鈥檛 quite as simple as one-temperature-fits-all. 鈥淓xperience has taught me that for my reagents, there鈥檚 not too much difference between -80 C and -65 C, which is what we鈥檙e using now,鈥� Reinhardt said. 鈥淏ut if you鈥檙e storing lots of messenger RNA, for example, your freezer might need to be colder because these reagents are the most sensitive to degradation.鈥�
For researchers working with samples that are difficult to acquire or take years to cultivate, the hesitation to change the temperature of their cold storage is understandable. This is why lab users around the world are working together to address this uncertainty by聽聽on how well specific reagents keep when stored in less cold 鈥� but still very, very cold 鈥� temperatures. Campaigns such as the聽initiative, led by longtime independent sustainable research consultant Allen Doyle, are also encouraging scientists not to associate a specific temperature with their freezers.
Turning up efforts鈥� and temperatures
This movement isn鈥檛 novel on 平特五不中 campuses either. In 2011, researchers in the Faculty of Medicine received support from the Sustainability Projects Fund (SPF) for a聽Green Biobanking聽project, which helped labs implement a safe and effective technique for storing DNA samples at room temperature as an alternative to long-term freezer storage.
鈥淭he reality is probably 80 to 90 per cent of the typical items stored in an ultra-low freezer are not being actively used and may never be,鈥� Reinhardt said. 鈥淪ince it takes so long to generate sophisticated reagents, you don鈥檛 just get rid of the unused materials. You store them because they could very well be used in future experiments.鈥� As well as minimizing the energy used to keep research 鈥榣eftovers鈥�, 鈥渋t鈥檚 very important to have an accurate inventory of what is in the freezer and periodically review it,鈥� he added, to avoid losing track of samples due to high user turnover in the lab.
These sustainable lab practices and others are all steps that lab users can take as part of the聽. This year, 平特五不中 is even offering its own prizes for labs that participate in the free competition, which has helped save more than 24 million kWh of energy worldwide since its creation six years ago.
Sign your lab up聽for the International Freezer Challenge and find 平特五不中-specific resources and tips for how to take action on the聽Office of Sustainability website.